Optimized Crosslinking of Gelatin Microspheres with Fructose
Keywords:
Gelatin, Crosslinking, Fructose, Experimental PlanningAbstract
Crosslinking reactions are a way to minimize the solubility of gelatin in water and thus enable its application in drug delivery systems. In this paper the use of fructose as a crosslinker was studied in order to minimize the cytotoxic effect caused by petroleum derivates as glutaraldehyde. The crosslinking of gelatin with fructose was carried out by water-in-oil emulsion and microspheres were prepared without a surfactant utilization. The experimental parameters were optimized by a complete 23 factorial design, to study the effects of pH, emulsion heating time and fructose concentration. The variance analisys results showed that pH and emulsion heating time have statistical significance to gelatin crosslink while fructose concentration have not a greater influence in this propertie. The samples’ solubility declined when using high pH and long heating times, indicating greater crosslinking degree under these conditions. However thermogravimetric and amino group titration analisys revealed that gelatin microspheres prepared with pH=9, emulsion heating time of 60 min and fructose concentration of 100% (w/w) were the best experimental parameters to produce gelatin microspheres crosslinked with fructose under the conditions studied in this work.
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